For instance, if cells plated on a sheet of 10 mm in axial length are stretched 10%, the whole sheet elongates 1 mm, and thus the position of the cells may displace to a similar degree. In the case of using a single actuator for stretching, the position of cells of interest often shifts out of the field of view temporarily during stretching. Cell culture chambers supporting the sheet may be stretched on a microscope by using actuators such as a stepping motor. 4–13 For these observations, cells are typically seeded on an extensible silicone elastomer sheet. 1–3 Live cell imaging has been extensively performed to investigate stretch-induced changes in protein dynamics. Identical cells can thus be continuously observed during stretching, thereby potentially enabling imaging of stretch-triggered fast dynamics.Ĭells regulate their functions in response to physical stretch. Even under this small displacement within the filed of view, our device allows the cells to undergo physiologically relevant levels of stretch. The cell-supporting chamber is stretched on an inverted microscope by using a piezoelectric actuator that provides small, but precisely controlled displacements. Cells are plated in a custom-designed stretch chamber with an elastomer sheet of a small cell culture area. To circumvent this situation, here we describe a robust methodology in which these cell shifts are minimized. ![]() ![]() In this case, the position of the cells of interest tends to shift out of the field of view upon stretch, making real-time imaging of identical cells difficult. ![]() For live cell microscopy combined with stretch experiments, cells are typically seeded on an extensible elastomer sheet. Cellular response to physical stretch has been extensively studied as a regulator of various physiological functions.
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